Transferring free DNA liberated from the donor (bacteria) to the extracellular surroundings resulting in the assimilation and typically the expression of the acquired characteristics in the recipient bacteria.Bacterial transformation does not necessitate a living donor. The only requirement is the free DNA. Such recipients which propagate the newly found DNA successfully are referred to as the transformant. In harsh weather conditions, a few genes of bacteria impulsively give out DNA from the cells to the environment that is ready to accept through the competent cells. These cells in turn are responsive to modifications in the surroundings thereby regulating the degree of gene acquisition via a natural transformation phenomena.The basis of bacterial transformation is the natural tendency of the bacteria to liberate DNA that is further consumed by other competent bacteria whose success is dependent on the competence of such host cells. Those entities which are transformable (naturally) immediately release its DNA in the stationary phase through autolysis. Some bacteria such as E.coli are treated artificially in labs so as to raise its potential to transform through the usage of chemicals, or through the application of a strong electric field or through the usage of a heat shock.Electroporation causes an increase in the competence through an increase in the cell wall’s permeability allowing the donor DNA to enter. Likewise, the transformants are chosen if the transformed DNA contains a selectable marker namely the antimicrobial resistance. In other cases, the DNA encodes to utilize the growth factor for instance amino acid