{"type":"video","version":"1.0","width":1920,"height":1080,"title":"Reverse vaccinology. - Animated Video By Akhila Kanuganti - Mango Animate","description":"Reverse vaccinology. animation video uploaded by Akhila Kanuganti. Stage #1: input data gathering and preparationProteome retrievalsequences ideally fromall strains or species of target organismCreate core proteomeRemove proteins that are...paralogous or duplicatedhomologous to vaccine recipient sequences toxicallergenicprotein sequences of core proteome-Stage #2: predicting proteins exposed to immune system Classical reverse vaccinologyPredict informative protein characteristics...[Antigenicity] [Subcellular localisation] [Transmembrane domains] [Function] [Signal peptides] [Virulence] [Adhesion] [Physico-chemical properties]sequences of antigenic proteins1-Stage #3: predicting epitopes (immunoinformatics)Epitope selection based on...1 Helper T-lymphocyte (HTL) Cytotoxic T lymphocytes (CTL) B-cell epitopesbinding affinity epitope density population coverageVaccine constructStage #4: computationally verify vaccine construct- Check for... Antigenicity, Allergenicity, Toxicity, Solubility, StabilityProtein-protein interactionCodon optimization In silico cloning3D structure modelling Structure refinementMolecular docking with receptor Molecular dynamics simulation | Binding free energy calculationsimmune system simulationMulti-epitope Vaccine for laboratory testing. Make your animation and host online for free!","url":"https:\/\/mangoanimate.com\/w\/wl4epcmtwffckyy\/reverse-vaccinology\/wl2epbmt0fdckyz\/","author_name":"Akhila Kanuganti","author_url":"https:\/\/mangoanimate.com\/homepage\/1ef06275-3ee7-6034-83a3-f23c915625cf","provider_name":"Mango Animate","provider_url":"https:\/\/mangoanimate.com","thumbnail_url":"https:\/\/online.mangoanimate.com\/ai\/w\/123454498666390144\/1\/thumb.jpg","thumbnail_width":1920,"thumbnail_height":1080,"html":"<iframe src=\"https:\/\/mangoanimate.com\/w\/wl4epcmtwffckyy\/reverse-vaccinology\/wl2epbmt0fdckyz\/?type=embed\" width=\"840px\" height=\"473px\" frameborder=\"0\" scrolling=\"no\" webkitAllowFullScreen mozallowfullscreen allowFullScreen><\/iframe>"}